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Hasegawa, Noboru; Ochi, Yoshihiro; Kawachi, Tetsuya; Nishikino, Masaharu; Ishino, Masahiko; Imazono, Takashi; Kaihori, Takeshi; Sasaki, Akira; Terakawa, Kota*; Minami, Yasuo*; et al.
Proceedings of SPIE, Vol.8140, p.81400G_1 - 81400G_8, 2011/10
Times Cited Count:3 Percentile:82.12(Engineering, Electrical & Electronic)We have developed a soft X-ray laser (SXRL) interferometer capable of the single-shot imaging of nano-scaled structure dynamics. The interferometer consisted of the reflection optics including double Lloyd's mirrors and focusing optics, and the interference fringes are produced on the detector surface. By using this interferometer, the initial stage (50 ps) of the ablation process of the Pt surface pumped by a 70 fs Ti:Sapphire laser pulse was observed. The expansion speed of the surface estimated from the result (34 nm/50 ps) indicated that the nano-bubble structures were formed in the initial stage of the ablation. In order to observe the detailed dynamics, the temporal synchronization between the pump and probe pulses was improved to be 3 ps by adopting a portion of the SXRL and pump beams as the time fiducials, to which the pump and probe timing was adjusted by using the X-ray streak camera.
Pirozhkov, A. S.; Kando, Masaki; Esirkepov, T. Z.; Gallegos, P.*; Ahmed, H.*; Ragozin, E. N.*; Faenov, A. Ya.*; Pikuz, T.; Koga, J. K.; Kiriyama, Hiromitsu; et al.
Proceedings of SPIE, Vol.8140, p.81400A_1 - 81400A_16, 2011/10
Imazono, Takashi; Koike, Masato; Kawachi, Tetsuya; Koeda, Masaru*; Nagano, Tetsuya*; Sasai, Hiroyuki*; Oue, Yuki*; Yonezawa, Zeno*; Kuramoto, Satoshi*; Sano, Kazuo*
Proceedings of SPIE Europe Optics + Optoelectronics 2011, Vol.8139, p.81390V_1 - 81390V_9, 2011/09
Times Cited Count:3 Percentile:82.12(Optics)Ishino, Masahiko; Kado, Masataka; Shinohara, Kunio*; Yamamoto, Yoshimasa*; Hirai, Itaru*; Kishimoto, Maki; Nishikino, Masaharu; Hasegawa, Noboru; Tamotsu, Satoshi*; Yasuda, Keiko*; et al.
Proceedings of SPIE Europe Optics + Optoelectronics 2011, Vol.8139, p.81390R_1 - 81390R_8, 2011/09
Times Cited Count:0 Percentile:0.01(Optics)Ultra thin gold films are favorable laser plasma targets for a soft X-ray microscopy, because the thin films emit intense soft X-rays at the wavelength of water window region. Using rear side emissions, the distance between the X-ray source and the specimens can be reduced so that the X-ray flux on specimens increases. The microscope system can be designed to be compact when the specimen holder and X-ray source are combined in one piece. The biological specimen holder combined with an ultra thin film target has been developed. This X-ray microscope system needs not any X-ray optics which causes a decrease in X-ray photons for imaging. X-ray images of hydrated living cells have been obtained successfully by use of the newly developed specimen holder. Specimen holder combined with plasma X-ray source will be a key component of a compact soft X-ray microscope using in a laboratory.
Kado, Masataka; Ishino, Masahiko; Kishimoto, Maki; Tamotsu, Satoshi*; Yasuda, Keiko*; Kinjo, Yasuhito*; Shinohara, Kunio*
Proceedings of SPIE Europe Optics + Optoelectronics 2011, Vol.8139, p.81390O_1 - 81390O_7, 2011/09
Laser plasma X-ray sources have high intensity and short pulse duration, and are suitable for X-ray microscopy in biology. They make wet live biological specimens possible to be imaged with a single shot X-ray exposure and several works have been done to image them. However there were no reports on the imaging of fine structures of cellular organelles in a live biological cell since higher X-ray intensity is needed for it. We have developed a high intensity laser plasma X-ray source, cooperating it with contact X-ray microscopy, and observed fine structures of cellular organelles in a wet biological cells. Comparing the X-ray images and the fluorescence images of cellular organelles such as actin filaments and mitochondria we have been clearly able to identify organelles in the X-ray images and observed fine structures.